ABSTRACT
Objective: To observe the inhibitory effect of combination of disulfiram (DSF) and cisplatin (CDDP) on the proliferation of triple-negative breast cancer (TNBC) MDA-MB-231 cells, and to explore its possible mechanism. Methods: The MDA-MB-231 cells in the logarithmic phase were divided into blank control group. DSF group. CDDP group and combination groups (60/xmol • L 1 CDDP+1. 2 and 4/imol • L ' DSF). MTT method was used to detect the survival rates of the MDA-MB-231 cells in various groups and flow cytometry was used to detect the apoptotic rates of the MDA-MB-231 cells in various groups, flow cytometry was used to detect the level of active oxygen ( ROS ) in the MDA-MB-231 cells in various groups, and Inductively Coupled Plasma-MassSpectrometry (ICP-MS) was used to detect the levels of Pt in the MDA-MB-231 cells in various groups. Results: The MTT method and flow cytometry results showed that after treated for 72 h. compared with 60/xmol • L ' CDDPgroup, the survival rates of the MDA-MB-231 cells in combination 1 group (60/xmol • L CDDP+1/xmol • L 1 DSF), combination 2 group (60/xmol • L 'CDDP+ 2/xmol • L 1 DSF) and combination 3 group (60/xmol • L 'CDDP+4/xmol • L 'DSF) were decreased ( P<0. 05). After treated for 24 h. compared with 60/x mol • L ' CDDP group and 2/x mol • L DSF group, the apoptotic rate of the MDA-MB-231 cells in combination group (60/xmol • L 'CDDP+2/xmol • L ' DSF) was increased ( P<0. 05). Compared with CDDP group, the level of ROS in the MDA-MB-231 cells in combination group (60/xmol • L 'CDDP+2/xmol • L 1 DSF) was increased (P<0. 05). The 1CP-MS results showed that compared with CDDP group, the level of Pt in the MDA-MB-231 cells in combination group (20/xmol • L 1CDDP+ 0.625/xmol • L ' DSF) was increased (P< 0.05). Conclusion: The combination of DSF and CDDP can significantly inhibit the proliferation of MDA-MB-231 cells, and its possible mechanism may be related to inhibiting the growth of tumor cells by increasing the ROS level and Pt content in the MDA-MB-231 cells.